anti pstat3 s727 (Cell Signaling Technology Inc)
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Anti Pstat3 S727, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pstat3+s727/pm41874002-195-53-55
Average 86 stars, based on 1 article reviews
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Western Blot:Article Title: STAT3 S727 phosphorylation drives pathogenic Th17 differentiation and neuroinflammation in autoimmune disease Article Snippet: Proteins were transferred to PVDF membranes using a BioRad Trans-Blot Turbo transfer system with the preprogrammed mixed molecular weight setting. .. Antibodies used for Western blotting purchased from Cell Signaling Technology included: Article Title: STAT3 S727 phosphorylation drives pathogenic Th17 differentiation and neuroinflammation in autoimmune disease. Article Snippet: Proteins were transferred to PVDF membranes using a BioRad Trans-Blot Turbo transfer system with the preprogrammed mixed molecular weight setting. .. Antibodies used for Western blotting purchased from Cell Signaling Technology included: other:Article Title: Semaphorin7A and PD-L1 cooperatively drive immunosuppression during mammary involution and breast cancer. Article Snippet: Membranes were washed with 1X Tris-buffered saline with Tween 20, incubated with a goat anti-rabbit HRP secondary antibody (Abcam; Cat# 6721), and developed with enhanced luminescence detection system (Thermo Fisher; Cat# 32106) on the Licor Odyssey. Article Title: Inhibiting the Otub1/phosphorylated STAT3 axis for the treatment of non-small cell lung cancer. Article Snippet: The transcription factor STAT3 is a promising target for the treatment of non-small cell lung cancer (NSCLC).. STAT3 activity is mainly dependent on phosphorylation at tyrosine 705 (pSTAT3-Y705), but the modulation on pSTAT3-Y705 is elusive.. By screening a library of deubiquitinases (Dubs), we found that the Otub1 increases STAT3 transcriptional activity. Expressing:Article Title: Sucrose solution, but not liquid sucrose diet, leads to leptin resistance irrespective of the time of day that sucrose is available Article Snippet: Following blocking in 5% milk the membrane was exposed to primary antibody overnight at 4°C, washed twice with Tris-0.5% TWEEN and once with Tris buffer, incubated for 1 h with goat HRP-anti-rabbit or anti-mouse antibody (111–035-003 or 115–035-003 tackson ImmunoResearch Laboratories, Inc., West Grove, PA), washed with Tris buffers, exposed to ECL reagent (ECL Select, Amersham, Little Chalfont, UK) and imaged for detection of protein (Azure Biosystems C600, Dublin, CA). .. Samples were analyzed for expression of pSTAT3(Y705) (9145, Cell Signaling Technologies Inc, Danvers, MA), pSTAT3( |
