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anti pstat3 s727  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti pstat3 s727
    Anti Pstat3 S727, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pstat3+s727/pm41874002-195-53-55
    Average 86 stars, based on 1 article reviews
    anti pstat3 s727 - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Western Blot:

    Article Title: STAT3 S727 phosphorylation drives pathogenic Th17 differentiation and neuroinflammation in autoimmune disease
    Article Snippet: Proteins were transferred to PVDF membranes using a BioRad Trans-Blot Turbo transfer system with the preprogrammed mixed molecular weight setting. .. Antibodies used for Western blotting purchased from Cell Signaling Technology included: pSTAT3 S727 (CAS#9134), pSTAT3 Y705 (CAS#9145), STAT3 (CAS#12640), pSTAT1 Y701 (CAS#9167), STAT1 (CAS#9172), T-bet (CAS#97135), NFAT (CAS#8032), NPM (CAS#3542), β-Tubulin (CAS#5346), β-actin (CAS#5125). .. For the cytoplasmatic and nuclear extraction, cells were cultured for 4 days and the fractions were extracted according to the manufacturer’s protocol (Thermo Scientific; CAS: 78833).

    Article Title: STAT3 S727 phosphorylation drives pathogenic Th17 differentiation and neuroinflammation in autoimmune disease.
    Article Snippet: Proteins were transferred to PVDF membranes using a BioRad Trans-Blot Turbo transfer system with the preprogrammed mixed molecular weight setting. .. Antibodies used for Western blotting purchased from Cell Signaling Technology included: pSTAT3 S727 (CAS#9134), pSTAT3 Y705 (CAS#9145), STAT3 (CAS#12640), pSTAT1 Y701 (CAS#9167), STAT1 (CAS#9172), T-bet (CAS#97135), NFAT (CAS#8032), NPM (CAS#3542), β-Tubulin (CAS#5346), β-actin (CAS#5125). .. For the cytoplasmatic and nuclear extraction, cells were cultured for 4 days and the fractions were extracted according to the manufacturer’s protocol (Thermo Scientific; CAS: 78833).

    other:

    Article Title: Semaphorin7A and PD-L1 cooperatively drive immunosuppression during mammary involution and breast cancer.
    Article Snippet: Membranes were washed with 1X Tris-buffered saline with Tween 20, incubated with a goat anti-rabbit HRP secondary antibody (Abcam; Cat# 6721), and developed with enhanced luminescence detection system (Thermo Fisher; Cat# 32106) on the Licor Odyssey.

    Article Title: Inhibiting the Otub1/phosphorylated STAT3 axis for the treatment of non-small cell lung cancer.
    Article Snippet: The transcription factor STAT3 is a promising target for the treatment of non-small cell lung cancer (NSCLC).. STAT3 activity is mainly dependent on phosphorylation at tyrosine 705 (pSTAT3-Y705), but the modulation on pSTAT3-Y705 is elusive.. By screening a library of deubiquitinases (Dubs), we found that the Otub1 increases STAT3 transcriptional activity.

    Expressing:

    Article Title: Sucrose solution, but not liquid sucrose diet, leads to leptin resistance irrespective of the time of day that sucrose is available
    Article Snippet: Following blocking in 5% milk the membrane was exposed to primary antibody overnight at 4°C, washed twice with Tris-0.5% TWEEN and once with Tris buffer, incubated for 1 h with goat HRP-anti-rabbit or anti-mouse antibody (111–035-003 or 115–035-003 tackson ImmunoResearch Laboratories, Inc., West Grove, PA), washed with Tris buffers, exposed to ECL reagent (ECL Select, Amersham, Little Chalfont, UK) and imaged for detection of protein (Azure Biosystems C600, Dublin, CA). .. Samples were analyzed for expression of pSTAT3(Y705) (9145, Cell Signaling Technologies Inc, Danvers, MA), pSTAT3(S727) (9134, Cell Signaling Technologies), total STAT3 (4904, Cell Signaling Technologies), O -GlcNAc (ab2739, Abcam, Cambridge, UK), O-linked N-acetylglucosaminyltransferase (OGT: ab96718, Abcam), glutamine fructose-6-phosphate amidotransferase (GFAT: 5322, Cell Signaling Technologies) and O -GlcNAcase (OGA: 147–11-1-AP, Proteintech Group, Rosemont, IL). ..



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    Cell Signaling Technology Inc rabbit monoclonal anti pstat3 s727 antibody
    Sialin interacts with <t>pSTAT3</t> <t>S727</t> and promotes its mitochondrial translocation in MSCs. a Gene Ontology biological process (GO-BP) enrichment analysis of the differentially phosphorylated proteins. b Dual-view hierarchical graph learning model predicts the interaction between Sialin and candidate proteins. c Co-immunoprecipitation (Co-IP) showing the interaction between Sialin and pSTAT3 S727 in hMSCs. d Surface plasmon resonance (SPR) analysis confirming the direct binding between Sialin and pSTAT3 S727 in hMSCs (KD = 683 nmol/L). e IF staining images and quantitation of pSTAT3 S727 colocalization with mitochondria by Pearson’s correlation coefficient in control, Sialin-overexpressing (HA- SLC17A5 ), and mitochondria-targeted Sialin-overexpressing (HA-Mito- SLC17A5 ) hMSCs. Scale bars, 10 μm. f A schematic model: Sialin binds pSTAT3 S727 , promotes its translocation to mitochondria, and thereby activates mitochondrial function in hMSCs. Data are presented as the mean ± SD, n = 3, except for f ( n = 30). **** P < 0.000 1
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    Cell Signaling Technology Inc pstat3 s727
    Sialin interacts with <t>pSTAT3</t> <t>S727</t> and promotes its mitochondrial translocation in MSCs. a Gene Ontology biological process (GO-BP) enrichment analysis of the differentially phosphorylated proteins. b Dual-view hierarchical graph learning model predicts the interaction between Sialin and candidate proteins. c Co-immunoprecipitation (Co-IP) showing the interaction between Sialin and pSTAT3 S727 in hMSCs. d Surface plasmon resonance (SPR) analysis confirming the direct binding between Sialin and pSTAT3 S727 in hMSCs (KD = 683 nmol/L). e IF staining images and quantitation of pSTAT3 S727 colocalization with mitochondria by Pearson’s correlation coefficient in control, Sialin-overexpressing (HA- SLC17A5 ), and mitochondria-targeted Sialin-overexpressing (HA-Mito- SLC17A5 ) hMSCs. Scale bars, 10 μm. f A schematic model: Sialin binds pSTAT3 S727 , promotes its translocation to mitochondria, and thereby activates mitochondrial function in hMSCs. Data are presented as the mean ± SD, n = 3, except for f ( n = 30). **** P < 0.000 1
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    https://www.bioz.com/product/pstat3+s727/pm41152886-209-11-7
    Average 86 stars, based on 1 article reviews
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    Image Search Results


    Sialin interacts with pSTAT3 S727 and promotes its mitochondrial translocation in MSCs. a Gene Ontology biological process (GO-BP) enrichment analysis of the differentially phosphorylated proteins. b Dual-view hierarchical graph learning model predicts the interaction between Sialin and candidate proteins. c Co-immunoprecipitation (Co-IP) showing the interaction between Sialin and pSTAT3 S727 in hMSCs. d Surface plasmon resonance (SPR) analysis confirming the direct binding between Sialin and pSTAT3 S727 in hMSCs (KD = 683 nmol/L). e IF staining images and quantitation of pSTAT3 S727 colocalization with mitochondria by Pearson’s correlation coefficient in control, Sialin-overexpressing (HA- SLC17A5 ), and mitochondria-targeted Sialin-overexpressing (HA-Mito- SLC17A5 ) hMSCs. Scale bars, 10 μm. f A schematic model: Sialin binds pSTAT3 S727 , promotes its translocation to mitochondria, and thereby activates mitochondrial function in hMSCs. Data are presented as the mean ± SD, n = 3, except for f ( n = 30). **** P < 0.000 1

    Journal: Bone Research

    Article Title: Sialin-STAT3 axis regulates bone homeostasis in mice

    doi: 10.1038/s41413-025-00504-2

    Figure Lengend Snippet: Sialin interacts with pSTAT3 S727 and promotes its mitochondrial translocation in MSCs. a Gene Ontology biological process (GO-BP) enrichment analysis of the differentially phosphorylated proteins. b Dual-view hierarchical graph learning model predicts the interaction between Sialin and candidate proteins. c Co-immunoprecipitation (Co-IP) showing the interaction between Sialin and pSTAT3 S727 in hMSCs. d Surface plasmon resonance (SPR) analysis confirming the direct binding between Sialin and pSTAT3 S727 in hMSCs (KD = 683 nmol/L). e IF staining images and quantitation of pSTAT3 S727 colocalization with mitochondria by Pearson’s correlation coefficient in control, Sialin-overexpressing (HA- SLC17A5 ), and mitochondria-targeted Sialin-overexpressing (HA-Mito- SLC17A5 ) hMSCs. Scale bars, 10 μm. f A schematic model: Sialin binds pSTAT3 S727 , promotes its translocation to mitochondria, and thereby activates mitochondrial function in hMSCs. Data are presented as the mean ± SD, n = 3, except for f ( n = 30). **** P < 0.000 1

    Article Snippet: Proteins were incubated with IgG (control), Sialin, or pSTAT3 S727 conjugated Dynabeads (Life Technologies) at room temperature for 4 h. Following several antibody tests, the rabbit polyclonal anti-Sialin antibody from Thermo and the rabbit monoclonal anti-pSTAT3 S727 antibody from CST yielded efficient immunoprecipitation results.

    Techniques: Translocation Assay, Immunoprecipitation, Co-Immunoprecipitation Assay, SPR Assay, Binding Assay, Staining, Quantitation Assay, Control